Comparative evaluation of 5'-end-sequence quality of clones in CAP trapper and other full-length-cDNA libraries.
作者： Yuichi Sugahara , Piero Carninci , Masayoshi Itoh , Kazuhiro Shibata , Hideaki Konno
DOI: 10.1016/S0378-1119(00)00557-6
关键词: cDNA library 、 Genetics 、 Complementary DNA 、 Reverse transcriptase 、 Expressed sequence tag 、 Library 、 Sequence alignment 、 Genomic library 、 Gene 、 Biology
摘要: To enhance the usefulness of laboratory mouse and to facilitate rapid assay gene functions we have been collecting entire set full-length cDNA by one-pass sequencing. collect clones efficiently, it is critical construct high-quality libraries. In recent years, developing a way libraries using biotinylation cap structure (the 'CAP-trapper' method) coupled with treatment increase reverse transcriptase efficiency at high temperature addition trehalose. this paper report our evaluation quality CAP trapper number other libraries, including results 5' end analysis in We used procedure that compared 5'-ends those genes public databases. Our showed 63% had sequences were either same length as equivalent database or 5'-extended, 90% these maintained their coding sequences. These indicate library promising tool for large-scale projects. Comparison full-length-cDNA confirmed value
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