Generation and analysis of 280,000 human expressed sequence tags.
作者： Ladeana Hillier , Greg Lennon , Michael Becker , M Fatima Bonaldo , Brandi Chiapelli
DOI: 10.1101/GR.6.9.807
关键词:
摘要: We report the generation of 319,311 single-pass sequencing reactions (known as expressed sequence tags, or ESTs) obtained from 5' and 3' ends 194,031 human cDNA clones. Our goal has been to obtain tag sequences many different genes deposit these in publicly accessible Data Base for Expressed Sequence Tags. Highly efficient automatic screening data allows deposition annotated without delay. Sequences have generated 26 oligo(dT) primed directionally cloned libraries, which 18 were normalized. The libraries constructed using mRNA isolated 17 tissues representing three developmental states. Comparisons a subset our with nonredundant protein bases show that ESTs represent known contain are novel. Analysis families Hidden Markov Models confirms this observation supports contention although normalization reduces significantly relative abundance redundant clones, it does not result complete removal members gene families.
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