Transformation of plant pathogenic fungi

摘要: The ability to introduce exogenous DNA into living cells and demonstrate its expression is a requirement for many research programs whose goals include determining the function of specific genes. Plant pathogenic fungi are highly appropriate subjects transformation experiments, because number fungal genes have been identified products believed, but not proven, play significant roles in interactions with host plants. For example, enzymes which detoxify antimicrobial compounds produced by plants response infection believed be important pathogenicity some [46]; degrade cell wall components appear role [22]; Phytotoxins hypothesized serve as or virulence factors [49]. Isolation encoding these [40, 50], followed their vitro mutagenesis reintroduction will permit conclusive analysis disease. Transformation general plant particular has advanced rapidly recent years. This chapter presents brief survey progress field, discussion various methods available fungi, detailed protocol protoplasts.