TRANSFORMATION OF TRICHODERMA SPP. WITH PLASMIDS CONFERRING HYGROMYCIN B RESISTANCE
作者： A. Sivan , T. E. Stasz , M. Hemmat , C. K. Hayes , G. E. Harman
DOI: 10.1080/00275514.1992.12026194
关键词:
摘要: Several plasmids with different promoter sequences, all containing the HygB gene that confers resistance to antibiotic hygromycin B, were evaluated for their ability transform Trichoderma viride strain T 105-227 Nic-. Plasmid pH IB, which contains a sequence from Cochliobolus heterostrophus, was found give largest number of putative transformants. Transformation T. harzianum strains T12-2 His- and T95-1 Lys- attempted, time at transformed protoplasts exposed B be critical. If added before had regenerated least 6 h, no transformants obtained. However, if regeneration proceeded more than 12 many nontransformed formed thalli. Putative heterokaryotic. Even thalli grown in presence nuclei low; only 0.5 0.8% conidia capable producing B. Subcultures derived single B-resistant stable. Southern analysis indicated plasmid integrated into genome either tandem repeat pattern or copy insertion.
jstor.org 
sci-hub.se 参考文章(24)
Robert C. Garber, B. Gillian Turgeon, Olen C. Yoder, Transformation of plant pathogenic fungi Plant Molecular Biology Manual. pp. 195- 207 ,(1989) , 10.1007/978-94-009-0951-9_10
Grant A. Bitter, Kevin M. Egan, Raymond A. Koski, Matthew O. Jones, Steven G. Elliott, James C. Giffin, [33] Expression and secretion vectors for yeast Methods in Enzymology. ,vol. 153, pp. 516- 544 ,(1987) , 10.1016/0076-6879(87)53076-2
B G Turgeon, R C Garber, O C Yoder, Development of a fungal transformation system based on selection of sequences with promoter activity. Molecular and Cellular Biology. ,vol. 7, pp. 3297- 3305 ,(1987) , 10.1128/MCB.7.9.3297
W. Timberlake, M. Marshall, Genetic Engineering of Filamentous Fungi Science. ,vol. 244, pp. 1313- 1317 ,(1989) , 10.1126/SCIENCE.2525275
Jonathan Knowles, P�ivi Lehtovaara, Merja Penttil�, Tuula Teeri, Anu Harkki, Irma Salovuori, THE CELLULASE GENES OF TRICHODERMA Antonie Van Leeuwenhoek International Journal of General and Molecular Microbiology. ,vol. 53, pp. 335- 341 ,(1987) , 10.1007/BF00400557
GE Harman, AG Taylor, TE Stasz, Combining Effective Strains of Trichoderma harzianum and Solid Matrix Priming to Improve Biological Seed Treatments Plant Disease. ,vol. 73, pp. 631- 637 ,(1989) , 10.1094/PD-73-0631
Kevin R. Kaster, Stanley G. Burgett, Thomas D. Ingolia, Hygromycin B resistance as dominant selectable marker in yeast. Current Genetics. ,vol. 8, pp. 353- 358 ,(1984) , 10.1007/BF00419824
J. Wang, D. W. Holden, S. A. Leong, Gene transfer system for the phytopathogenic fungus Ustilago maydis Proceedings of the National Academy of Sciences of the United States of America. ,vol. 85, pp. 865- 869 ,(1988) , 10.1073/PNAS.85.3.865
Linda Gritz, Julian Davies, Plasmid-encoded hygromycin B resistance: the sequence of hygromycin B phosphotransferase gene and its expression in Escherichia coli and Saccharomyces cerevisiae Gene. ,vol. 25, pp. 179- 188 ,(1983) , 10.1016/0378-1119(83)90223-8
Andrew P. Feinberg, Bert Vogelstein, A technique for radiolabeling DNA restriction endonuclease fragments to high specific activity Analytical Biochemistry. ,vol. 132, pp. 6- 13 ,(1983) , 10.1016/0003-2697(83)90418-9