Isolation, sequencing, and disruption of the yeast CKA2 gene: casein kinase II is essential for viability in Saccharomyces cerevisiae.

摘要: Casein kinase II of Saccharomyces cerevisiae contains two distinct catalytic subunits, alpha and alpha', which are encoded by the CKA1 CKA2 genes, respectively. Null mutations in gene do not confer a detectable phenotype (J. L.-P. Chen-Wu, R. Padmanabha, C. V. Glover, Mol. Cell. Biol. 8:4981-4990, 1988), presumably because presence gene. We report here cloning, sequencing, disruption The alpha' subunit is 60% identical to CKA1-encoded 55% Drosophila (A. Saxena, 7:3409-3417, 1987). Deletions were constructed replacement techniques. Haploid cells alone disrupted show no phenotype, but haploid carrying disruptions both genes inviable. Cells casein activity depleted increase substantially size prior growth arrest, significant fraction arrested exhibit pseudomycelial morphology. Disruption also results flocculation. Yeast strains lacking endogenous can be rescued expression beta subunits or alone, suggesting that function has been conserved through evolution.