Protein Phosphatase 2C of Toxoplasma Gondii Interacts with Human SSRP1 and Negatively Regulates Cell Apoptosis.

摘要: Abstract Objective The protozoan Toxoplasma gondii expresses large amounts of a 37 kDa Type 2C serine-threonine phosphatase, the so-called TgPP2C which has been suggested to contribute parasite growth regulation. Ectopic expression in mammalian cells also indicated that enzyme could regulate and survival. In this study, we aimed investigate interaction with human SSRP1 (structure-specific recognition protein 1) effects on cell viability. Methods yeast two hybrid system, His-tag pull-down co-immunoprecipitation assays were used confirm determine binding domain SSRP1. evaluation apoptosis was performed using cleaved caspase-3 antibody Annexin-V/PI kit combined flow cytometry. Results We identified as an interacting partner TgPP2C. C-terminal region including amino acids 471 538 specifically mapped responsible for overexpression down-regulated negatively regulated induced by DRB, casein kinase II (CKII) inhibitor, through enhanced Conclusion may be parasitic factor capable promoting survival host SSRP1, thereby creating favorable environment growth.