Aberrant Production of Th1/Th2/Th17-Related Cytokines in Serum of C57BL/6 Mice after Short-Term Formaldehyde Exposure

摘要: Previous studies have shown that formaldehyde (FA) could cause immunotoxicity by changing the number of T lymphocytes and cytokines play a pivotal role in regulation lymphocytes. However, previously used cytokine detection methods are difficult to use measurement several small amount sample for one test. Therefore, cytometric bead array (CBA) technique was used. CBA showed better analytical efficiency sensitivity than previous methods. C57BL/6 mice were exposed control (normal saline), low FA concentration (0.5 mg/kg), high (2 mg/kg) 1 week or month. The contents cytokines, including Th1-related (IL-2, IFN-γ, tumor necrosis factor), Th2-related (IL-4, IL-6, IL-10), Th17-related (IL-17A), measured using BD FACS Canto II Flow Cytometer analyzed FCAP ArrayTM Software. Th1/Th2/Th17-related slightly decreasing trend after exposure. Conversely, significantly increasing found all serve important functions immune reactions