The Effects of Anti-LAP Monoclonal Antibody Down-regulation of CD4+LAP+ T Cells on Allogeneic Corneal Transplantation in Mice.

摘要: CD4+latency-associated peptide (LAP)+ T cells are a newly discovered cell subset with suppressive function on immune responses. In this study, we investigate the role of CD4+LAP+ mice corneal allograft survival by down-regulating their expression using anti-LAP mAb. We show that blockage LAP leads to decrease in percentage expressing CD4+Foxp3+, CD4+GARP+, and CD4+IL-10+ lymph nodes spleens undergoing orthotopic penetrating transplantation allograft, without affecting graft survival. addition, higher percentages CD4+IFN-γ+ CD4+IL-17A+ spleens, as well TNF, IFN-γ, IL-17A IL-6 levels aqueous humor, significantly increase rejected grafts. The TGF-β1 decreases grafts during rejection period. It is therefore possible mAb can down-regulate regulatory subsets its immunosuppressive effects. seems mainly be associated up-regulation Th1 Th17 peripheral nodes.