Solvent environment modulates effects of glutaraldehyde crosslinking on tissue-derived biomaterials.

摘要: Bioprosthetic materials utilized in the construction of heart valves and vascular grafts possess limited performance viability vivo. This is due (in part) to failure these mimic mechanical properties host tissue they replace. If bioprosthetic could be engineered meet required vivo, functional lifetime implants would increased. In this study, glutaraldehyde/solvent solutions decreasing dielectric constant (polarity) were modify crosslinked collagen whole bovine pericardial tissue. Solvents included phosphate buffer, methanol, 95% (w/w) ethanol, n-propanol, n-butanol. Exogenous crosslinking was verified by thermal denaturation tests amino acid analyses. Tensile behavior collagenous samples found depend upon employed; however, treatment solvents alone had little, if any, effect. As decreased, three systematically altered: plastic strain fell from a mean 8.9 +/- 1.5% (buffer) 1.6 0.4% (n-butanol); at fracture increased 32.2 2.6% 55.6 4.6% percent stress remaining after 1000-s relaxation an 80-g initial load 86.3 1.1% 76.9 1.0% (n-butanol). Crosslinking using glutaraldehyde/n-butanol solution produced with tensile that very close fresh tissue; flexural treated different those decoupling behaviors unique form treatment. The observed phenomena may results conformational changes facilitated polar/nonpolar interactions solvent are "locked in" action glutaraldehyde. technique aid "customized" design tissue-derived biomaterials.