Dynamics of Dnmt1 methyltransferase expression and intracellular localization during oogenesis and preimplantation development.
作者： Sarayu Ratnam , Carmen Mertineit , Feng Ding , Carina Y. Howell , Hugh J. Clarke
关键词: Regulation of gene expression 、 DNMT1 、 Genomic imprinting 、 Embryo 、 Methyltransferase 、 Biology 、 Molecular biology 、 DNA methylation 、 DNA methyltransferase 、 Methylation
摘要: The imprinting of mammalian genes depends on the maintenance DNA methylation patterns during pre- and postimplantation development. Dnmt1o is a variant form somatically expressed Dnmt1 cytosine methyltransferase that synthesized stored in oocyte cytoplasm trafficks to eight-cell nucleus preimplantation development, where it maintains alleles imprinted genes. Transcripts encoding are present embryos, suggesting protein also embryo, may account for at stages other than embryo. However, using an antibody detects Dnmt1, but not Dnmt1o, no was detected immunoblots or by immunocytochemical staining wildtype embryos. Moreover, produced from modified allele, Dnmt1(1s/1o), trafficked nuclei stages. highly restricted nuclear localization oocyte-derived development add further support notion methyltransferases required maintaining imprints
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sci-hub.se 参考文章(30)
Brigid Hogan, Elizabeth Lacy, Frank Costantini, Manipulating the mouse embryo: A laboratory manual ,(1986)
Michael R. Rountree, Kurtis E. Bachman, Stephen B. Baylin, DNMT1 binds HDAC2 and a new co-repressor, DMAP1, to form a complex at replication foci Nature Genetics. ,vol. 25, pp. 269- 277 ,(2000) , 10.1038/77023
Yong-Kook Kang, Deog-Bon Koo, Jung-Sun Park, Young-Hee Choi, An-Sik Chung, Kyung-Kwang Lee, Yong-Mahn Han, Aberrant methylation of donor genome in cloned bovine embryos. Nature Genetics. ,vol. 28, pp. 173- 177 ,(2001) , 10.1038/88903
Jing Li, Chris Papadopoulos, Jimmy Xu, Growing Y-junction carbon nanotubes Nature. ,vol. 402, pp. 253- 254 ,(1999) , 10.1038/46214
Masaki Okano, Shaoping Xie, En Li, Cloning and characterization of a family of novel mammalian DNA (cytosine-5) methyltransferases Nature Genetics. ,vol. 19, pp. 219- 220 ,(1998) , 10.1038/890
John J. Eppig, Evelyn E. Telfer, Isolation and culture of oocytes. Methods in Enzymology. ,vol. 225, pp. 77- 84 ,(1993) , 10.1016/0076-6879(93)25008-P
Guo-Liang Xu, Timothy H. Bestor, Déborah Bourc'his, Chih-Lin Hsieh, Niels Tommerup, Merete Bugge, Maj Hulten, Xiaoyan Qu, James J. Russo, Evani Viegas-Péquignot, Chromosome instability and immunodeficiency syndrome caused by mutations in a DNA methyltransferase gene. Nature. ,vol. 402, pp. 187- 191 ,(1999) , 10.1038/46052
H.J. Clarke, Y. Masui, J. Rossant, Suppression of chromosome condensation during meiotic maturation induces parthenogenetic development of mouse oocytes. Development. ,vol. 104, pp. 97- 103 ,(1988)
D. Humpherys, Epigenetic Instability in ES Cells and Cloned Mice Science. ,vol. 293, pp. 95- 97 ,(2001) , 10.1126/SCIENCE.1061402
Keiko Ohsawa, Yoshinori Imai, Daisuke Ito, Shinichi Kohsaka, Molecular Cloning and Characterization of Annexin V-Binding Proteins with Highly Hydrophilic Peptide Structure Journal of Neurochemistry. ,vol. 67, pp. 89- 97 ,(2002) , 10.1046/J.1471-4159.1996.67010089.X