Transcriptional regulation of type I collagen genes in cultured fibroblasts by a factor isolated from thioacetamide-induced fibrotic rat liver.

摘要: Abstract Recently Hatahara and Seyer (Hatahara, T., Seyer, J.M. Biochim. Biophys. Acta (1982) 716, 377-382) isolated a factor from fibrotic rat liver which stimulates collagen synthesis in cultured fibroblasts without affecting their rate of proliferation. To investigate the mechanism fibrogenic factor-mediated enhancement type I synthesis, we quantitated levels mRNAs coding for pro-alpha 1(I) 2(I) chains dermal fibroblasts. Cell-free translation experiments revealed that caused greater than 5-fold increase translatable mRNAs. We also by techniques Northern blotting glyoxylated poly(A+) RNA followed hybridization to nick-translated human cDNA clones containing sequence chains. Furthermore, investigated relative rates mRNA transcription nuclei treated control Similar quantitation beta-actin transcription, remains unaffected treatment with factor, was used as an internal control. demonstrate causes 4-6-fold genes. Finally, show intracellular degradation is not significantly altered cells factor. These results combined data on cell-free strongly suggest increased accumulation factor-treated consequence enhanced transcription.