Physical and gene mapping of chloroplast DNA from Atriplex triangularis and Cucumis sativa

摘要: A rapid and simple method for constructing restriction maps of large DNAs (100-200 kb) is presented. The utility this illustrated by mapping the Sal I, Sac Hpa I sites 152 kb Atriplex triangularis chloroplast genome, Pvu II 155 Cucumis sativa genome. These two are very similar in organization; both feature near-universal DNA inverted repeat sequence 22-25 kb. positions four different genes have been localized on these DNAs. In genomes 16S 23S ribosomal RNAs encoded duplicate situated at one end repeat, while subunit ribulose-1,5-bisphosphate carboxylase a 32 kilodalton photosystem polypeptide separated 55 within single copy region. physical genetic organization compared to that spinach DNA.