Organelle DNA isolation and RFLP analysis

摘要: The analysis of restriction fragment length polymorphisms (RFLPs) in organelle DNA is radically different from that nuclear both purpose and execution. fundamental reason for these differences genome size: land plants, chloroplast (cpDNA) 1-2 x 102 kb size [38, 39] mitochondrial (mtDNA) 2-20 [14, 26, 37, 39], whereas most genomes are 1-10 106 [2]. larger reflected our relatively poor understanding their structure the fact only a small fraction genes have been isolated analyzed. Physical mapping RFLPs an entire, average-sized forbidding task whose completion at least several years away, physical even 1 % region presents significant challenge. As consequence, presently used primarily as genetic markers linkage studies aimed identifying selecting traits agronomic importance [27, 44] examining modes chromosomal evolution [15, 54]. Nuclear just beginning to be chromosome walking efforts clone mapped interest [58].