Membrane shape modulates transmembrane protein distribution.
作者： Sophie Aimon , Andrew Callan-Jones , Alice Berthaud , Mathieu Pinot , Gilman E.S. Toombes
DOI: 10.1016/J.DEVCEL.2013.12.012
关键词: Membrane curvature 、 Peripheral membrane protein 、 Biology 、 Elasticity of cell membranes 、 Membrane protein 、 Biological membrane 、 Cell biology 、 Transmembrane channels 、 Membrane transport 、 Membrane fluidity
摘要: Although membrane shape varies greatly throughout the cell, contribution of curvature to transmembrane protein targeting is unknown because numerous sorting mechanisms that take place concurrently in cells. To isolate effect shape, we used cell-sized giant unilamellar vesicles (GUVs) containing either potassium channel KvAP or water AQP0 to form nanotubes with controlled radii. Whereas concentrations flat and curved membranes were indistinguishable, was enriched tubes, greater enrichment more highly membranes. Fluorescence recovery after photobleaching measurements showed both proteins could freely diffuse through neck between tube GUV, each on stiffness characterized using a thermodynamic model. This study establishes importance for transmembrane provides method determining effective flexibility proteins.
core.ac.uk
sci-hub.se 参考文章(38)
Helen C. Lai, Lily Y. Jan, The distribution and targeting of neuronal voltage-gated ion channels Nature Reviews Neuroscience. ,vol. 7, pp. 548- 562 ,(2006) , 10.1038/NRN1938
D. R. Daniels, M. S. Turner, Diffusion on membrane tubes : a highly discriminatory test of the Saffman-Delbruck theory Langmuir. ,vol. 23, pp. 6667- 6670 ,(2007) , 10.1021/LA0635000
V.S. Markin, Lateral organization of membranes and cell shapes Biophysical Journal. ,vol. 36, pp. 1- 19 ,(1981) , 10.1016/S0006-3495(81)84713-3
Sarah L. Veatch, Pietro Cicuta, Prabuddha Sengupta, Aurelia Honerkamp-Smith, David Holowka, Barbara Baird, Critical fluctuations in plasma membrane vesicles. ACS Chemical Biology. ,vol. 3, pp. 287- 293 ,(2008) , 10.1021/CB800012X
U Valentin Nägerl, Katrin I Willig, Birka Hein, Stefan W Hell, Tobias Bonhoeffer, None, Live-cell imaging of dendritic spines by STED microscopy. Proceedings of the National Academy of Sciences of the United States of America. ,vol. 105, pp. 18982- 18987 ,(2008) , 10.1073/PNAS.0810028105
J. Hu, Y. Shibata, C. Voss, T. Shemesh, Z. Li, M. Coughlin, M. M. Kozlov, T. A. Rapoport, W. A. Prinz, Membrane proteins of the endoplasmic reticulum induce high-curvature tubules. Science. ,vol. 319, pp. 1247- 1250 ,(2008) , 10.1126/SCIENCE.1153634
Sophie Aimon, John Manzi, Daniel Schmidt, Jose Antonio Poveda Larrosa, Patricia Bassereau, Gilman E. S. Toombes, Functional Reconstitution of a Voltage-Gated Potassium Channel in Giant Unilamellar Vesicles PLoS ONE. ,vol. 6, pp. e25529- ,(2011) , 10.1371/JOURNAL.PONE.0025529
M. C. Ashby, S. R. Maier, A. Nishimune, J. M. Henley, Lateral Diffusion Drives Constitutive Exchange of AMPA Receptors at Dendritic Spines and Is Regulated by Spine Morphology The Journal of Neuroscience. ,vol. 26, pp. 7046- 7055 ,(2006) , 10.1523/JNEUROSCI.1235-06.2006
B. Sorre, A. Callan-Jones, J. Manzi, B. Goud, J. Prost, P. Bassereau, A. Roux, Nature of curvature coupling of amphiphysin with membranes depends on its bound density Proceedings of the National Academy of Sciences of the United States of America. ,vol. 109, pp. 173- 178 ,(2012) , 10.1073/PNAS.1103594108
Pankaj Singh, Paritosh Mahata, Tobias Baumgart, Sovan Lal Das, Curvature sorting of proteins on a cylindrical lipid membrane tether connected to a reservoir. Physical Review E. ,vol. 85, pp. 051906- 051906 ,(2012) , 10.1103/PHYSREVE.85.051906