Development of an integrated optical analyzer for characterization of growth dynamics of bacterial colonies.

作者: Huisung Kim , Nan Bai , Arun K. Bhunia , Galen B. King , E. Daniel Hirleman

DOI: 10.1002/JBIO.201200224

关键词: Bacterial colonyAspect ratioDynamics (mechanics)Bacterial colony morphologyAnalytical chemistryConfocalOptical densitySpectrum analyzerBiology

摘要: In order to understand the biophysics behind collective behavior of a bacterial colony, confocal displacement meter was used measure profiles colonies, together with custom built optical density circuits. The system delivered essential information related quantitative growth dynamics (height, diameter, aspect ratio, density) colony. For example, ratio S. aureus approximately two times higher than that E. coli O157 : H7, while OD 1/3 O157 : H7. (© 2013 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim)

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