Determination of Microvessel Density by Quantitative Real-time PCR in Esophageal Cancer: Correlation with Histologic Methods, Angiogenic Growth Factor Expression, and Lymph Node Metastasis

摘要: Purpose: Angiogenesis and lymphangiogenesis are important steps in tumor growth dissemination of prognostic importance solid tumors. The determination microvessel density (MVD) by immunohistology is subject to considerable variability between different laboratories observers. We compared MVD quantitative real-time PCR correlated the results with clinical variables. Experimental Design: expression endothelial antigens vascular cadherin (CD144), P1H12 (CD146), tie-2, VEGFR-2, lymphatic markers VEGFR-3, Prox, LYVE was assessed (qPCR) primary surgical samples. angiogenetic factors VEGF-A, VEGF-C, VEGF-D, angiopoietin-1, angiopoietin-2 quantified Results: VEGFR-2 each other 54 samples esophageal cancer ( P Conclusions: These indicate that quantification qPCR carcinoma yields similar immunohistology. Interestingly, extent angiogenesis not related individual Lymph node metastases could be predicted VEGF-C expression.