Cloning and 5' end nucleotide sequences of two juvenile hormone-inducible vitellogenin genes of the African migratory locust.
作者： JOHN LOCKE , BRADLEY N. WHITE , GERARD R. WYATT
关键词: Repeated sequence 、 DNA sequencing 、 Exon 、 Molecular biology 、 genomic DNA 、 Biology 、 Gene 、 Southern blot 、 Coding region 、 Intron 、 Genetics
摘要: Screening of lambda libraries prepared with locust (Locusta migratoria) genomic DNA yielded overlapping clones containing two complete vitellogenin genes, designated A and B, along extensive 3'- 5'-flanking sequences. Genes B are about 12 kb 10.5 long, respectively, both hybridize on Northern blots 6300-nucleotide RNAs previously identified as mRNAs. Each gene contains a large intron near the 5' end must also contain other introns. Repetitive "Lm1 elements," similar to human Alu repetitive elements, repeated sequences occur within in regions each gene. Southern showed no cross-hybridization between major portions mRNA-coding genes B. have been determined for terminal exons flanking genes. Both short (56-58 nucleotide) leader exons, which, unlike rest coding region, highly conserved sequence (87% identical) code amino acids that resemble amino-terminal vertebrate vitellogenins. The upstream several stretches similarity may be involved juvenile hormone regulated expression.
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