Chemistry of antibody binding to a protein
作者： H. Geysen , J. Tainer , S. Rodda , T. Mason , H Alexander
关键词: Antigenicity 、 Peptide 、 Bound water 、 Stereochemistry 、 Antigen 、 Protein secondary structure 、 Molecule 、 Chemistry 、 Epitope 、 Protein sequencing
摘要: The chemistry of antibody recognition was studied by mapping the antigenicity protein myohemerythrin with peptide homologs sequence. results suggest that entire surface is antigenic, but probability there being antibodies to a given site influenced local stereochemistry. Although accessible an binding domain, least reactive positions cluster in most tightly packed and mobile regions are closely associated narrow, concave grooves molecular containing bound water molecules. frequently recognized sites form three-dimensional superassemblies characterized high mobility, convex shape, often negative electrostatic potential.
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Frederic M. Richards, Calculation of molecular volumes and areas for structures of known geometry Methods in Enzymology. ,vol. 115, pp. 440- 464 ,(1985) , 10.1016/0076-6879(85)15032-9
R Jemmerson, Y Paterson, Mapping epitopes on a protein antigen by the proteolysis of antigen-antibody complexes Science. ,vol. 232, pp. 1001- 1004 ,(1986) , 10.1126/SCIENCE.2422757
Elizabeth D. Getzoff, John A. Tainer, Paul K. Weiner, Peter A. Kollman, Jane S. Richardson, David C. Richardson, Electrostatic recognition between superoxide and copper, zinc superoxide dismutase Nature. ,vol. 306, pp. 287- 290 ,(1983) , 10.1038/306287A0
Robert J.P. Williams, Geoffrey R. Moore, Protein antigenicity, organization and mobility Trends in Biochemical Sciences. ,vol. 10, pp. 96- 97 ,(1985) , 10.1016/0968-0004(85)90258-0
E. Getzoff, H. Geysen, S. Rodda, H Alexander, J. Tainer, R. Lerner, Mechanisms of antibody binding to a protein Science. ,vol. 235, pp. 1191- 1196 ,(1987) , 10.1126/SCIENCE.3823879
A. Amit, R. Mariuzza, S. Phillips, R. Poljak, Three-dimensional structure of an antigen-antibody complex at 2.8 A resolution Science. ,vol. 233, pp. 747- 753 ,(1986) , 10.1126/SCIENCE.2426778
D. J. Barlow, M. S. Edwards, J. M. Thornton, Continuous and discontinuous protein antigenic determinants Nature. ,vol. 322, pp. 747- 748 ,(1986) , 10.1038/322747A0
Scott J. Weiner, Peter A. Kollman, David A. Case, U. Chandra Singh, Caterina Ghio, Guliano Alagona, Salvatore Profeta, Paul Weiner, A NEW FORCE FIELD FOR MOLECULAR MECHANICAL SIMULATION OF NUCLEIC ACIDS AND PROTEINS Journal of the American Chemical Society. ,vol. 106, pp. 765- 784 ,(1984) , 10.1021/JA00315A051
Cyrus Chothia, Joël Janin, Principles of protein–protein recognition Nature. ,vol. 256, pp. 705- 708 ,(1975) , 10.1038/256705A0
A. Shrake, J.A. Rupley, Environment and exposure to solvent of protein atoms. Lysozyme and insulin. Journal of Molecular Biology. ,vol. 79, pp. 351- 371 ,(1973) , 10.1016/0022-2836(73)90011-9