Simultaneous infection of human host with genetically distinct isolates of Paracoccidioides brasiliensis

作者: João Batista Júnior , Rodrigo Berzaghi , Adelita Duanra Medeiros do Monte Arnaud , Cor Jesus Fernandes Fontes , Zoilo Pires de Camargo

DOI: 10.1590/S0074-02762010000100009

关键词: MicrobiologyHost (biology)Paracoccidioides brasiliensisParacoccidioidomycosisGenetic diversityAnatomical sitesGeneticsGenetic variabilityRAPDBiologyGenotype

摘要: This study is the first report on genetic differences between isolates of Paracoccidioides brasiliensis from a single patient. We describe simultaneous infection with genetically distinct P. in patient chronic paracoccidioidomycosis. The clinical were obtained lesions different anatomical sites and characterised by random amplified polymorphic DNA (RAPD) analysis. RAPD technique can be helpful for distinguishing isolates. Different primers used to characterise these patterns allowed differentiation construction phenetic tree, which showed more than 28% variability this fungal species, opening new possibilities studies brasiliensis. Based results preliminary findings, we suggest that genotypes might infect same patient, inducing active form disease.

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