Metabolism under Microaerobic Conditions of Mitochondria from Cowpea Nodules
作者： Stephen Rawsthorne , Thomas A. Larue
DOI: 10.1104/PP.81.4.1097
关键词: Cyanide 、 Malonate 、 Biochemistry 、 Respiration 、 Metabolism 、 Mitochondrion 、 Biology 、 Fumarate reductase 、 Adenosine triphosphate 、 Leghemoglobin 、 Plant science 、 Genetics 、 Physiology
摘要: A method is described for isolating mitochondria from nodules of cowpea (Vigna unguiculata [L.] Walp.) under completely anaerobic conditions. The were immediately active when incubated aerobically with substrates, and their respiration rates higher than prepared in air. lacked fumarate reductase not inhibited by 5% CO2. When microaerobic conditions, could be measured leghemoglobin spectroscopy. Microaerobic was approximately 50% 1 millimolar malonate, cyanide. O2 uptake the ATP/O ratio declined therefore ATP production may low environment infected nodule cells.
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