Uncoupler-Resistant Glucose Uptake by the Thermophilic Glycolytic Anaerobe Thermoanaerobacter thermosulfuricus (Clostridium thermohydrosulfuricum).

摘要: The transport of glucose across the bacterial cell membrane Thermoanaerobacter thermosulfuricus (Clostridium thermohydrosulfuricum) Rt8.B1 was governed by a permease which did not catalyze concomitant substrate and phosphorylation thus phosphoenolpyruvate-dependent phosphotransferase. Glucose uptake carrier mediated, could be driven an artificial potential (Δψ) in presence or absence sodium, sensitive to inhibitors dissipate proton motive force (Δp; tetrachlorosalicylanilide, N,N-dicyclohexylcarboiimide, 2,4-dinitrophenol), no nonmetabolizable analog 2-deoxyglucose demonstrated. glucokinase apparent Km for (0.21 mM) similar Kt (affinity constant) (0.15 mM), suggesting that controls rate uptake. Inhibitors ATP synthesis (iodoacetate sodium fluoride) also inhibited uptake, this effect due reduction level available phosphorylation. These results indicated T. lacks concentrative system is via facilitated diffusion, followed ATP-dependent glucokinase. In Rt8.B1, metabolized Embden-Meyerhof-Parnas pathway, yields 2 mol (G. M. Cook, unpublished data). Since only 1 used glucose, energetics are therefore those found bacteria possess