Decreased androstenedione production with increased follicular maturation in theca cells from the domestic hen (Gallus domesticus).

摘要: Collagenase-dispersed theca cells from the 3rd and 4th largest ovarian follicles (T3) were responsive to LH stimulation of both oestrogen androstenedione production, whereas follicle (T1) failed respond gonadotrophin stimulation. Similarly, 8-bromo cAMP forskolin more effective in stimulating production T3 than T1 cells, indicating that post-receptor events involved decreased responsiveness cells. The C17-20-lyase activity, as measured by conversion [3H]17-hydroxyprogesterone androstenedione, was greatly reduced compared results demonstrate a decrease addition aromatase contributes loss LH-stimulated steroidogenesis mature