Changes in esterase and superoxide dismutase isozymes during in vitro morphogenesis in Plantago ovata Forssk

摘要: Callus cultures were established from hypocotyl explants of Plantago ovata in Murashige and Skoog's medium supplemented with 2,4-d/Kinetin NAA/BA combinations. Calluses growing on (0.4 mg l−1 each) regenerated into plantlets after the second subculture when transferred to media containing IAA (0.2 l−1) BA (5 l−1). Shoot tip multiplication was carried out same BA. Tissue samples calluses, regenerating multiplying shoot tips grown vitro extracted protein extraction buffer subjected esterase superoxide dismutase isozyme analysis. The calluses however, showed a uniform banding even different hormone shootlets two new bands which not found either control or plants. A band also analysed for dismutase. It is postulated that those enzyme forms arise as well may de novo due post-transcriptional modification genes are essential ovata.