Synthesis, characterization, photocleavage, cytotoxicity in vitro, apoptosis, and cell cycle arrest of ruthenium(II) complexes
作者： Qi-Feng Guo , Si-Hong Liu , Qing-Hua Liu , Hui-Hua Xu , Jian-Hua Zhao
DOI: 10.1080/00958972.2012.680592
关键词: Flow cytometry 、 Ruthenium 、 Cell cycle 、 Intercalation (chemistry) 、 Cytotoxicity 、 Chemistry 、 Bromide 、 Molecular biology 、 Cleavage (embryo) 、 Apoptosis 、 Medicinal chemistry
摘要: Two new ruthenium(II) complexes, [Ru(dmp)2(BHIP)]2+ (1) and [Ru(dmb)2(BHIP)]2+ (2), were synthesized characterized by elemental analysis, ESI-MS, 1H NMR. DNA-binding constants of these complexes with calf-thymus DNA (ct-DNA) determined to be 2.09 (±0.18) × 104 (mol L−1)−1 (s = 2.58) 1.48 (±0.17) × 105 (s = 1.57), respectively. Viscosity measurements show that 1 2 interact ct-DNA intercalation. Upon irradiation at 365 nm, induce cleavage pBR322 DNA. The cytotoxicity was evaluated 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. apoptosis induced the studied flow cytometry. results cell cycle arrest can inhibit proliferation BEL-7402 cells in G0/G1 phase.
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