Three-dimensional measurement of cerebral microvascular plasma perfusion, glial fibrillary acidic protein and microtubule associated protein-2 immunoreactivity after embolic stroke in rats: a double fluorescent labeled laser-scanning confocal microscopic study.
作者： Zheng Gang Zhang , Lucille Bower , Rui Lan Zhang , Song Chen , Joseph P Windham
DOI: 10.1016/S0006-8993(99)01886-7
关键词: Microtubule-associated protein 2 、 Ischemia 、 Central nervous system 、 Biology 、 Pathology 、 Perfusion 、 Microcirculation 、 Fluorescein isothiocyanate 、 Cortex (anatomy) 、 Glial fibrillary acidic protein
摘要: Early astroglial response to post-ischemic microvascular hypoperfusion may contribute progressive cerebral microcirculatory impairment and ischemic neuronal injury. Using laser-scanning confocal microscopy three fluorescent probes, we measured in three-dimensions plasma perfusion, astrocytic reactivity, injury assessed by fluorescein isothiocyanate (FITC)-dextran, GFAP immunoreactivity, microtubule associated protein-2 (MAP2) respectively, rats subjected 2 h of middle artery occlusion. Three-dimensional quantitative analysis revealed that embolic ischemia resulted a significant (P<0.05) reduction perfusion the ipsilateral cortex subcortex. Tissue within subcortex with low exhibited increase immunoreactivity compared homologous contralateral tissue. re-constructed images showed prominent immunoreactive astrocytes surrounded large vessels decreased downstream capillaries MCA territory when Triple fluorescence probe-stained sections tissue increased was accompanied MAP2 immunoreactivity. The present study demonstrates an induces early reactive further perfusion. three-dimensional imaging used provides means investigate parenchymal cellular responses changes after
elsevier.com
sci-hub.se 参考文章(36)
Eng Lf, DeArmond Sj, Glial fibrillary acidic (GFA) protein immunocytochemistry in development and neuropathology. Progress in Clinical and Biological Research. pp. 65- 79 ,(1981)
G. J. del Zoppo, Microvascular changes during cerebral ischemia and reperfusion. Cerebrovascular and brain metabolism reviews. ,vol. 6, pp. 47- 96 ,(1994)
Oliver S. Kempski, C. Volk, Neuron-Glial Interaction During Injury and Edema of the CNS Acta Neurochirurgica. ,vol. 60, pp. 7- 11 ,(1994) , 10.1007/978-3-7091-9334-1_2
Carol K. Petito, Marilda Chung, Issam A. Halaby, Arthur J.L. Cooper, Influence of the neuronal environment on the pattern of reactive astrocytosis following cerebral ischemia. Progress in Brain Research. ,vol. 94, pp. 381- 387 ,(1992) , 10.1016/S0079-6123(08)61766-5
R. L. Wright, J. M. Thurston, rd A. Ames, G. Majno, M. Kowada, Cerebral ischemia. II. The no-reflow phenomenon. American Journal of Pathology. ,vol. 52, pp. 437- 453 ,(1968)
J. H. Garcia, Jinying Lian, Y. Yoshida, Kai-Feng Liu, Song Chen, Brain microvessels: factors altering their patency after the occlusion of a middle cerebral artery (Wistar rat). American Journal of Pathology. ,vol. 145, pp. 728- 740 ,(1994)
G. V. W. Johnson, R. S. Jope, The role of microtubule-associated protein 2 (MAP-2) in neuronal growth, plasticity, and degeneration Journal of Neuroscience Research. ,vol. 33, pp. 505- 512 ,(1992) , 10.1002/JNR.490330402
Hua Chen, Michael Chopp, Lonni Schultz, Gordon Bodzin, Julio H. Garcia, Sequential neuronal and astrocytic changes after transient middle cerebral artery occlusion in the rat Journal of the Neurological Sciences. ,vol. 118, pp. 109- 116 ,(1993) , 10.1016/0022-510X(93)90099-K
Deborah A. Dawson, Christl A. Ruetzler, John M. Hallenbeck, Temporal impairment of microcirculatory perfusion following focal cerebral ischemia in the spontaneously hypertensive rat. Brain Research. ,vol. 749, pp. 200- 208 ,(1997) , 10.1016/S0006-8993(96)01166-3
Wolfgang Walz, Artur Klimaszewski, I. Alick Paterson, Glial Swelling in Ischemia: A Hypothesis Developmental Neuroscience. ,vol. 15, pp. 216- 225 ,(1993) , 10.1159/000111337