Molecular Cytogenetic Aberrations in Tunisian Patients with Multiple Myeloma Identified by cIg-FISH in Fixed Bone Marrow Cells

摘要: Cytogenetic studies in multiple myeloma (MM) are hampered by the hypo-proliferative nature of plasma cells. In order to circumvent this problem, we have used a combination immunolabeling cytoplasmic Ig light chains (λ or κ) and FISH (cIg-FISH), which allowed comprehensive detection most common and/or recurrent molecular cytogenetic aberrations on fixed bone marrow cells 70 Tunisian patients. Translocations involving chromosome 14q32 region were observed 32 cases (45.7%), including 18 with t(11;14), 8 t(4;14), 2 t(14;16). Deletions 13q14 (D13S319/RB1) detected 18.6%, deletions 17p13 (TP53) 5.7% cases, respectively. Of all patients D13S319/RB1 deletion, 61.5% also carried translocation, whereas TP53 associated t(11;14) (50%) D13S319 deletion 1 case (25%). Our results suggest that there is correlation between presence translocations MM cIg-FISH more sensitive as compared conventional karyotyping detecting abnormalities disease.