Detection of 13q abnormalities in multiple myeloma using immunomagnetically selected plasma cells.

摘要: Accurate prognostic evaluation of patients with multiple myeloma (MM) is required for their stratification more adequate therapy. Chromosomal G-banding and interphase fluorescence in situ hybridization (FISH) on cell-nonspecific samples cells selected by magnetic-activated cell separation (MACS) were used to study 13 from 12 patients. Bone marrow (BM) analysed using three approaches. Standard mitotic prepared after G-banding. Interphase FISH was performed detect the 13q14 deletion unselected BM cells. In parallel, CD138-specific antibody. The high-purity suspension then deletion. Magnetic yielded enriched suspensions mean viability 98.0% (range: 97.0%-99.0%), purity 97.6% 87.2%-99.2%) as detected morphologically, 85.2% 44.8%-98.4%) immunophenotyping CD138+ revealed 13q14.3 5 (38.5%) 9 (69.2%) suspensions. conclusion, immunomagnetically MM increases detection MM.