Amplification of the 16S-23S Spacer Region in rRNA Operons of Mycoplasmas by the Polymerase Chain Reaction

摘要: Summary Nucleotide sequences of the spacer region between 16S and 23S DNA in ribosomal RNA operons mycoplasmas were identified by analysis products polymerase chain reaction (PCR) amplified from corresponding regions 12 species this family. Three common PCR primers, F1, F2, R1, designed similarity these sequences. Primers F1 R1 produced fragments 340 to 660 bp when each was used as template. Specific amplification confirmed a second round which templates F2 primers. No discrete band observed electrophoresis human or mouse served template with use primers R2, suggests that many mycoplasmal sometimes contaminate culture eukaryotic cells can be detected PCR.