Specificity and performance of PCR detection assays for microbial pathogens.

摘要: PCR has become a widely used tool for detection, identification and differentiation of pathogenic microorganisms in diagnosis animal human diseases. However, quite number currently protocols can be further optimized to exclude nonspecific reactions. On the one hand, target sequences as defined by primer binding sites should checked carefully absence significant homologies other organisms order insure high specificity detection. A major part assays is still based on ribosomal RNA operon, but, differentiating potential this region limited, genes encoding cellular proteins, such toxins, surface antigens or enzymes, have been shown viable alternative many instances. various approaches are available improve performance amplification reaction itself. The kinetics known heavily dependent primer-to-template ratio, efficiency annealing enzyme-to-template ratio. In present paper, recently published detection microorganisms, particularly bacterial pathogens, reviewed optimization strategies explained. practical implications epidemiological consequences routine use diagnostic laboratory also discussed.