Proteomic Analysis of Membrane Proteins from Streptococcus pneumoniae with Multiple Separation Methods Plus High Accuracy Mass Spectrometry
作者： Xuesong Sun , Xiao-Yan Yang , Xing-Feng Yin , Guangchuang Yu , Chuan-Le Xiao
关键词: Bacteria 、 Streptococcus pneumoniae 、 Secretion 、 Membrane protein 、 Membrane 、 Signal transduction 、 Proteomics 、 Protein folding 、 Biology 、 Biochemistry
摘要: … Protein samples were purified with 2-D Clean-Up Kit (GE Healthcare, Uppsala, Sweden) and resolubilized again in solubilization buffer. The protein concentration was determined …
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