Hsa_circ_0000231 knockdown inhibits the glycolysis and progression of colorectal cancer cells by regulating miR-502-5p/MYO6 axis

摘要: Colorectal cancer (CRC) poses a heavy threat to human health owing its high incidence and mortality. Circular RNAs (circRNAs) were investigated participate in the progression of CRC, whereas there was no revenant data on CRC process regulated by hsa_circ_0000231. This study aimed explore effects hsa_circ_0000231 underneath regulatory mechanism. The expression levels hsa_circ_0000231, miR-502-5p, Myosin VI (MYO6) mRNA detected quantitative real time polymerase chain reaction (qRT-PCR). Western blot employed determine protein MYO6 proliferating cell nuclear antigen (PCNA). proliferation, apoptosis, migration, invasive determined counting kit-8 proliferation (CCK-8) colony formation assays, flow cytometry analysis, wound-healing assay, transwell invasion respectively. Glucose uptake lactate production severally illustrated glucose assay kit kit. relationship between miR-502-5p or predicted circular RNA interactome targetScan online databases, identified dual-luciferase reporter immunoprecipitation (RIP) assays. In vivo tumor carried out knockdown growth vivo. Hsa_circ_0000231 dramatically upregulated while obviously downregulated tissues cells compared with control groups. repressed PCNA protein. Functionally, glycolysis, migration invasion, induced these decreased inhibitor. Mechanistically, acted as sponge bound MYO6. Furthermore, volume weight inhibited glycolysis downregulating through sponging which might provide theoretical basis further studying circ_0000231-directed therapy CRC.