Decrease of dynamic range of proteins in human plasma by ampholine immobilized polymer microspheres
作者： Nan Deng , Guijie Zhu , Yuanbo Chen , Qi Wu , Zhen Liang
DOI: 10.1016/J.ACA.2014.04.004
关键词: Proteome 、 Polymer 、 Protein digestion 、 Blood proteins 、 Incubation 、 Digestion 、 Trifluoroacetic acid 、 Fractionation 、 Chemistry 、 Chromatography
摘要: A novel protein sample pretreatment method based on ampholine immobilized polymer microsphere (ampholine@PM) was developed for the fractionation of intact proteins prior to digestion and peptide analysis reduce dynamic range human plasma proteome. After incubation with our prepared ampholine@PM, captured were successively desorbed by 2 M NaCl, 100 mM glycine-hydrochloric acid, 30% (v/v) acetonitrile 0.1% trifluoroacetic acid. The SDS-PAGE results showed in such three fractions obviously reduced as compared native plasma. On-particle ultimately performed release all retained ampholine@PM. Followed MuPIT analysis, number identified improved 75% after ampholine@PM treatment. Furthermore, spectral count 9 high abundance decreased 37.6-97.2%, low (< ng mL(-1)) increased from 4 17. These demonstrated that could efficiently decrease abundance, beneficial achieve deep coverage identification (c) 2014 Elsevier B. V. All rights reserved.
irgrid.ac.cn参考文章(56)
Chengjian Tu, Jun Li, Rebeccah Young, Brian J. Page, Frank Engler, Marc S. Halfon, John M. Canty, Jun Qu, Combinatorial Peptide Ligand Library Treatment Followed by a Dual-Enzyme, Dual-Activation Approach on a Nanoflow Liquid Chromatography/Orbitrap/Electron Transfer Dissociation System for Comprehensive Analysis of Swine Plasma Proteome Analytical Chemistry. ,vol. 83, pp. 4802- 4813 ,(2011) , 10.1021/AC200376M
Mary Kathryn Doud, Michael W Schmidt, David Hines, Claudia Naumann, Andreas Kocourek, Noushin Kashani-Poor, Robert Zeidler, Dieter A Wolf, Rapid prefractionation of complex protein lysates with centrifugal membrane adsorber units improves the resolving power of 2D-PAGE-based proteome analysis BMC Genomics. ,vol. 5, pp. 25- 25 ,(2004) , 10.1186/1471-2164-5-25
Mats Jonsson, Stig Fredriksson, Mats Jontell, Anders Linde, Isoelectric focusing of the phosphoprotein of rat-incisor dentin in ampholine and acid pH gradients Journal of Chromatography A. ,vol. 157, pp. 235- 242 ,(1978) , 10.1016/S0021-9673(00)92338-0
Pier Giorgio Righetti, Elisabetta Gianazza, Isoelectric focusing of heparin: Evidence for complexing with carrier ampholytes Biochimica et Biophysica Acta. ,vol. 532, pp. 137- 146 ,(1978) , 10.1016/0005-2795(78)90456-7
Subhashini Selvaraju, Ziad El Rassi, Reduction of protein concentration range difference followed by multicolumn fractionation prior to 2-DE and LC-MS/MS profiling of serum proteins ELECTROPHORESIS. ,vol. 32, pp. 674- 685 ,(2011) , 10.1002/ELPS.201000606
Ming-xia Gao, Jin Hong, Peng-yuan Yang, Xing-min Zhang, Chromatographic prefractionation prior to two-dimensional electrophoresis and mass spectrometry identifies : Application to the complex proteome analysis in rat liver Analytica Chimica Acta. ,vol. 553, pp. 83- 92 ,(2005) , 10.1016/J.ACA.2005.07.060
Silvia Surinova, Ralph Schiess, Ruth Hüttenhain, Ferdinando Cerciello, Bernd Wollscheid, Ruedi Aebersold, On the Development of Plasma Protein Biomarkers Journal of Proteome Research. ,vol. 10, pp. 5- 16 ,(2011) , 10.1021/PR1008515
Thierry Rabilloud, Cécile Lelong, Mireille Chevallet, Sylvie Luche, Two-dimensional gel electrophoresis in proteomics: Past, present and future. Journal of Proteomics. ,vol. 73, pp. 2064- 2077 ,(2010) , 10.1016/J.JPROT.2010.05.016
Laura Santucci, Giovanni Candiano, Andrea Petretto, Chiara Lavarello, Maurizio Bruschi, Gian Marco Ghiggeri, Attilio Citterio, Pier Giorgio Righetti, Combinatorial ligand libraries as a two-dimensional method for proteome analysis Journal of Chromatography A. ,vol. 1297, pp. 106- 112 ,(2013) , 10.1016/J.CHROMA.2013.04.065
S. P. Gygi, G. L. Corthals, Y. Zhang, Y. Rochon, R. Aebersold, Evaluation of two-dimensional gel electrophoresis-based proteome analysis technology Proceedings of the National Academy of Sciences of the United States of America. ,vol. 97, pp. 9390- 9395 ,(2000) , 10.1073/PNAS.160270797