Rapid prefractionation of complex protein lysates with centrifugal membrane adsorber units improves the resolving power of 2D-PAGE-based proteome analysis
作者： Mary Kathryn Doud , Michael W Schmidt , David Hines , Claudia Naumann , Andreas Kocourek
关键词: Membrane 、 Fungal protein 、 Complex protein 、 Proteome 、 Gel electrophoresis 、 Chromatography 、 Proteomics 、 Biology 、 Chemical fractionation 、 Molecular biology
摘要: Background Two-dimensional gel electrophoresis (2D-PAGE) has proven over the years to be a reliable and efficient method for separation of hundreds proteins based on charge mass. Nevertheless, complexity even simplest proteomes limits resolving power 2D-PAGE. This limitation can partially alleviated by sample prefractionation using variety techniques.
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