Mesenchymal stromal cells mediate a switch to alternatively activated monocytes/macrophages after acute myocardial infarction

摘要: Given the established anti-inflammatory properties of mesenchymal stromal cells (MSCs), we investigated their effect on inflammatory cell infiltration ischemic cardiac tissue and function. We employed two types MSCs, human bone marrow-derived (BM) MSCs umbilical cord perivascular in an experimental acute myocardial infarction (MI) model with immune-deficient NOD/SCID gamma null mouse. Cells were infused 48 h after induction MI mice assessed 24 later (72 MI) for marrow (BM), circulating tissue-infiltrating monocytes/macrophages. showed that presence either MSC type, overall macrophage/monocyte levels reduced, including pro-inflammatory M1-type macrophages, while proportion alternatively activated M2-type macrophages was significantly increased circulation heart but not BM. Moreover, found decreased expression IL-1β IL-6, IL-10 fewer apoptotic cardiomyocytes without changes angiogenesis infarct area. Fractional shortening enhanced 2 weeks infusion similar to medium controls 16 MI. In vitro studies BM frequency monocytes/macrophages, part by MSC-mediated secretion IL-10. Our data suggest a new mechanism enhancement function, possibly via mediated switch from at site. Additional are warranted confirming role augmenting effects regeneration.