Improved left ventricular function after transplantation of microspheres and fibroblasts in a rat model of myocardial infarction.

摘要: As a novel and promising therapeutic strategy for heart failure, the application of different cell types is subject increasing research interest. In this study we investigated effect several microspheres (uniform polystyrene microspheres, 10 µm diameter) transplanted 4 weeks after induction myocardial infarction in rat model. Eight intramyocardial fibroblasts left ventricular function was significantly improved as demonstrated by isolated studies (Langendorff) echocardiographic findings (LVDP 129 ± 32.9 mmHg, LVDP 119.2 24.1 fractional shortening (FS) 38.9 4.6%, FS 36.84 6.05%) contrast to injection macrophages or medium alone 67 22.6 75.9 24.8 29.16 8.7%, 27.2 7.2%, P < 0.05). Signals Bromodesoxy-Uridine (BrdU) labeled were detected infarcted areas. Microspheres recorded abundantly autofluorescence. Significantly more apoptotic cells observed areas macrophage (328.6 37.4 cells/mm2) (338.7 16.5 cells/mm2; 0.05) treated hearts compared microsphere (233.2 16.8 fibroblast (232.2 19.1 injected hearts. Neovascularization, reflected density CD 31 positive vessels infracted area, did not differ between four groups studied. The increased number (fibroblasts 94.7 7.1 cells/mm2, 82.2 3.0 56.02 9.93 46.35 9.03 suggests that underlying mechanism augmented might be based on inflammatory processes.