In vitro lifespan and senescent behaviour of human periosteal derived stem cells

摘要: Periosteum derived progenitor cells (PDPCs) represent promising mesenchymal stem (MSCs) for skeletal regeneration and to test bone cell based tissue engineering strategies. Most of regenerative medicine approaches on MSCs require a noteworthy amount that must be expanded in vitro prior their use. As culture expansion method may impact behaviour, we assessed the replicative metabolic capacity (nitric oxide production glucose consumption), senescence hallmarks PDPCs serially passaged as well expression selected genes specifically related early osteoblastic differentiation, remodelling stemness during PDPC sequential passaging. We also scouted Systems Biology approach examine elucidate experimental results through mathematical modelling silico simulations. subculture led progressive proliferative decline but, despite this, maintained almost constant activity. In vitro, senescent displayed typical "replicative senescence" features, involving p16 not p53 regulation this phenomenon. Gene analysis evidenced tendency sub-cultured increase involved resorption. The showed strict similarity between age-related changes, enabling definition an model mimicking behaviour terms nitric (NO) production. relationship NO passages could cutting edge index". Overall, our findings suggest possibility use early-passage local recruitment cells, whilst later suitable tool validate scaffolds intended elderly subjects.