Dual regulation of meiosis in yeast.

摘要: The two regulatory pathways appear to come together at the IME1 gene. It is clearly regulated by mating type and induced starvation as well. Overexpression of completely overcomes MAT defects but may not circumvent all nutritional control. Kassir et al. (1988) found that overexpression allowed sporulation in presence glucose nitrogen. They also have a meiotic level message temperature-sensitive cdc25 diploids shifted high temperature rich medium (Simchen Kassir, 1989). Smith Mitchell (1989) an early event (recombination) medium, later events did occur (i.e., they detected no spore formation). (personal communication) has suggested difference be due differences amount nitrogen present experiments. Thus, while it clear necessary positive regulator meiosis, responding both conditions, if sufficient. possible other genes are involved response starvation. One interpretation separate control exerted for starting with meiosis I. Much pathway allows yeast cells enter been determined. As case many sensory transduction pathways, initial signal yet known, nor nature proposed downstream phosphorylated effector. Given power molecular genetics, answers these questions seem attainable. Another area remains unclear between responses versus carbon source. Many experiments discussed above do address this question. strategies used utilized developmental decisions other, more complex eukaryotes. Certainly several gene products homologies mammalian systems. For example, human H-ras can substitute RAS; relationship sufficiently close dominant Ha-ras mutations inhibit CDC25 (Powers al., Furthermore, appropriate phenotype cells, suggesting CDC25-like protein. Although major components defined, mechanism RME1-IME transcriptional determined.(ABSTRACT TRUNCATED AT 400 WORDS)