Relationship between target cell recognition and temporal fluctuations in intracellular Ca2+ of human NK cells.

摘要: Temporal changes in intracellular Ca2+ concentration, [Ca2+]i, of resting human peripheral blood NK cells response to target cell binding were evaluated by flow cytometry. [Ca2+]i was significantly elevated PBL and purified bound NK-sensitive K562 HSB2 cells, but not those NK-resistant MD1 B-lymphoblastoid cells. Thus, a) the ability a elicit flux correlated with its sensitivity lysis b) adhesion alone sufficient stimulus for induction. Conjugates sorted onto agarose-coated slides on basis relative 19-hr single agarose cytotoxicity assays. In contrast basal levels more strongly as judged stability conjugates sort-related shear forces (p less than 0.01), frequently killed which they attached 0.05). fluctuations observed target-bound both presence absence extracellular Ca2+. influx release from internal stores appeared contribute triggered appropriate These results support hypothesis that such constitute an early signal flagging occurrence recognition.