Surgical stress impairs natural killer cell programming of tumor for lysis in patients with sarcomas and other solid tumors.

摘要: Background. Natural killer (NK) cells may provide a first line of defense against the metastatic implantation circulating tumor emboli. Because emboli are discharged systemically in patients undergoing solid resection, it is important to determine nature surgical-stress impairment perioperative NK cell cytotoxic function. Methods. The authors studied 85 surgical resection tumors, most whom had an abrupt and marked decrease cytotoxicity that was detectable within 18 hours resection. Results. This not caused by rapidly emerging suppressor (measured autologous effector mixing studies) or decreased frequency peripheral blood (assessed phenotypically morphologically). Instead, stress exerted direct “toxic” effect on could be localized specific phase lysis cycle. Tumor binding round were intact postoperatively single-cell assays). However, postbinding programming for sharply after surgery calcium pulse In addition, kinetics rate lytic slower (assayed target saturation kinetic chromium-51 release tests). Conclusions. These latter defects probably related deficiency because postprogramming maximal recycling capacity affected stress.