Genomic sequence of a mutant strain of Caenorhabditis elegans with an altered recombination pattern.

摘要: The original sequencing and annotation of the Caenorhabditis elegans genome along with recent advances in technology provide an exceptional opportunity for genomic analysis wild-type mutant strains. Using Illumina Genome Analyzer, we sequenced entire Rec-1, a strain that alters distribution meiotic crossovers without changing overall frequency. Rec-1 was derived from ethylmethane sulfonate (EMS)-treated strains, one which had high level transposable element mobility. Sequencing this provides to examine consequences on altering recombination events. MAQ software, 83% base pair sequence reads were aligned reference available at Wormbase, providing 21-fold coverage genome. software programs Slider, observed 1124 differences between Wormbase (WS190), 441 mutagenized (BC313) N2 (VC2010). most frequent base-substitution G:C A:T, 141 chromosomes I or X, 55 31 respectively. With data removed, no obvious pattern apparent. No major chromosomal rearrangements observed, but additional insertions elements detected. There are 11 extra copies Tc1, 8 Tc2 genome, likely remains past high-hopper activity progenitor strain. Our high-throughput able detect regions direct repeat sequences, deletions, elements, differences. A subset alterations affecting coding confirmed by independent approach using oligo array comparative hybridization. phenotype is alteration preferred position event other significant phenotypic consequences. In study, evidence mutator effect nucleotide attributable mutation.