Influence of modification next to the anticodon in tRNA on codon context sensitivity of translational suppression and accuracy.
作者： F Bouadloun , T Srichaiyo , L A Isaksson , G R Björk
DOI: 10.1128/JB.166.3.1022-1027.1986
关键词: Biochemistry 、 Start codon 、 Genetics 、 Codon usage bias 、 Synonymous substitution 、 Biology 、 Silent mutation 、 Transfer RNA 、 Open reading frame 、 Codon degeneracy 、 Translational efficiency
摘要: Effects on translation in vivo by modification deficiencies for 2-methylthio-N6-isopentenyladenosine (ms2i6A) (Escherichia coli) or 2-methylthio-N6-(4-hydroxyisopentenyl)adenosine (ms2io6A) (Salmonella typhimurium) tRNA were studied mutant strains. These hypermodified nucleosides are present the 3' side of anticodon (position 37) reading codons starting with uridine. In E. coli, translational error caused was strongly reduced case third-position misreading a tryptophan codon (UGG) particular context but not affected first-position an arginine (CGU) another context. Misreading UGA nonsense at two different positions dependent. The efficiencies some suppressors decreased tRNA-dependent manner. Suppressor which lacks ms2i6A-ms2io6A becomes more sensitive to Our results therefore indicate that, besides improving efficiency, ms2i6A37 and ms2io6A37 modifications also involved decreasing intrinsic sensitivity tRNA. Possible consequences regulation gene expression discussed.
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sci-hub.se 参考文章(42)
B S Vold, J M Lazar, A M Gray, Characterization of a deficiency of N6-(delta 2-isopentenyl)-2-methylthioadenosine in the Escherichia coli mutant trpX by use of antibodies to N6-(delta 2-isopentenyl)adenosine. Journal of Biological Chemistry. ,vol. 254, pp. 7362- 7367 ,(1979) , 10.1016/S0021-9258(18)50327-6
F. Bouadloun, D. Donner, C.G. Kurland, Codon-specific missense errors in vivo. The EMBO Journal. ,vol. 2, pp. 1351- 1356 ,(1983) , 10.1002/J.1460-2075.1983.TB01591.X
Henry J. Vogel, David M. Bonner, Acetylornithinase of Escherichia coli: partial purification and some properties. Journal of Biological Chemistry. ,vol. 218, pp. 97- 106 ,(1956) , 10.1016/S0021-9258(18)65874-0
F Winston, D Botstein, J H Miller, Characterization of amber and ochre suppressors in Salmonella typhimurium. Journal of Bacteriology. ,vol. 137, pp. 433- 439 ,(1979) , 10.1128/JB.137.1.433-439.1979
Helga Kersten, On the Biological Significance of Modified Nucleosides in tRNA Progress in Nucleic Acid Research and Molecular Biology. ,vol. 31, pp. 59- 114 ,(1984) , 10.1016/S0079-6603(08)60375-X
J U Ericson, G R Björk, Pleiotropic effects induced by modification deficiency next to the anticodon of tRNA from Salmonella typhimurium LT2. Journal of Bacteriology. ,vol. 166, pp. 1013- 1021 ,(1986) , 10.1128/JB.166.3.1013-1021.1986
R B Loftfield, D Vanderjagt, The frequency of errors in protein biosynthesis Biochemical Journal. ,vol. 128, pp. 1353- 1356 ,(1963) , 10.1042/BJ1281353
Sidney Altman, Sheldon I. Feinstein, Context effects on nonsense codon suppression in Escherichia coli. Genetics. ,vol. 88, pp. 201- 219 ,(1978) , 10.1093/GENETICS/88.2.201
Houssier Grosjean, Krikor Nicoghosian, Etienne Haumont, Dieter Söll, Robert Cedergren, Nucleotide sequences of two serine tRNAs with a GGA anticodon: the structure-function relationships in the serine family of E. coli tRNAs Nucleic Acids Research. ,vol. 13, pp. 5697- 5706 ,(1985) , 10.1093/NAR/13.15.5697
Emanuel J. Murgola, Frances T. Pagel, Kathryn A. Hijazi, Codon context effects in missense suppression Journal of Molecular Biology. ,vol. 175, pp. 19- 27 ,(1984) , 10.1016/0022-2836(84)90442-X