ATP-induced aggregates of tubulin rings.
作者： J.R. Zabrecky , R.D. Cole
DOI: 10.1016/S0021-9258(19)70230-0
关键词: GTP' 、 A-site 、 Ion exchange 、 Tubulin 、 Nucleotide 、 Bovine brain 、 Stereochemistry 、 Microtubule 、 Chemistry
摘要: Abstract Aggregates of double-walled rings (46 nm diameter) were formed upon warming (37 degrees C) ion exchange purified bovine brain tubulin (1.5 to 2.0 mg/ml) in the presence 1.0 mM ATP and 5.0 Mg2+. The formation aggregated was blocked by GDP (1.0 mM), but when GTP mM) added subsequently, block overcome. Warming simultaneously produced a mixture microtubules rings. preparations used demonstrated be devoid transphosphorylase, it is therefore clear that action induction not mediated transphosphorylation. These results are consistent with an interaction nucleotide at site distinct from previously described N- E-sites.
sci-hub.st 参考文章(11)
R H Swisher, M L Landt, F J Reithel, The molecular weight of, and evidence for two types of subunits in, the molybdenum-iron protein of Azotobacter vinelandii nitrogenase. Biochemical Journal. ,vol. 163, pp. 427- 432 ,(1977) , 10.1042/BJ1630427
D.J. Lundell, J.B. Howard, Isolation and sequences of the cysteinyl tryptic peptides from the MoFe-protein of Azotobacter vinelandii nitrogenase. Journal of Biological Chemistry. ,vol. 256, pp. 6385- 6391 ,(1981) , 10.1016/S0021-9258(19)69176-3
K Weber, M Osborn, The reliability of molecular weight determinations by dodecyl sulfate-polyacrylamide gel electrophoresis Journal of Biological Chemistry. ,vol. 244, pp. 4406- 4412 ,(1969) , 10.1016/S0021-9258(18)94333-4
J. T. Stasny, R. C. Burns, B. D. Korant, R. W. F. Hardy, ELECTRON MICROSCOPY OF THE MO-FE PROTEIN FROM AZOTOBACTER NITROGENASE Journal of Cell Biology. ,vol. 60, pp. 311- 316 ,(1974) , 10.1083/JCB.60.1.311
D. Kleiner, C. H. Chen, Physical and chemical properties of the nitrogenase proteins form Azotobacter vinelandii. Archives of Microbiology. ,vol. 98, pp. 93- 100 ,(1974) , 10.1007/BF00425272
H. Fleming, R. Haselkorn, Differentiation in Nostoc muscorum: Nitrogenase Is Synthesized in Heterocysts Proceedings of the National Academy of Sciences of the United States of America. ,vol. 70, pp. 2727- 2731 ,(1973) , 10.1073/PNAS.70.10.2727
Barbara K. Burgess, Deloria B. Jacobs, Edward I. Stiefel, Large-scale purification of high activity Azotobacter vinelandII nitrogenase. Biochimica et Biophysica Acta. ,vol. 614, pp. 196- 209 ,(1980) , 10.1016/0005-2744(80)90180-1
Milton L. Bruening, Leroy H. Wullstein, Controlled atmosphere technique for measurement of molecular nitrogen, nitric oxide, nitrous oxide, and oxygen by gas chromatography Environmental Science & Technology. ,vol. 8, pp. 72- 75 ,(1974) , 10.1021/ES60086A010
HENRY METZGER, MARVIN B. SHAPIRO, JAMES E. MOSIMANN, JAY E. VINTON, Assessment of compositional relatedness between proteins. Nature. ,vol. 219, pp. 1166- 1168 ,(1968) , 10.1038/2191166A0
D W Cleveland, S G Fischer, M W Kirschner, U K Laemmli, Peptide mapping by limited proteolysis in sodium dodecyl sulfate and analysis by gel electrophoresis. Journal of Biological Chemistry. ,vol. 252, pp. 1102- 1106 ,(1977) , 10.1016/S0021-9258(19)75212-0