作者: Per L. Gregersen , Anders B. Christensen , Jens Sommer-Knudsen , David B. Collinge
DOI: 10.1007/BF00019493
关键词: Amino acid 、 Open reading frame 、 Peptide sequence 、 Hordeum vulgare 、 Molecular biology 、 Biology 、 cDNA library 、 Gene 、 Southern blot 、 Nucleic acid sequence
摘要: A cDNA clone, pBH72-F1 (F1), was isolated from a library prepared barley leaves 72 h after inoculation with Erysiphe graminis f.sp. hordei. The 1388 bp nucleotide sequence of contains an open reading frame encoding 42.3 kDa polypeptide 390 amino acids which shows similarity to O-methyltransferases (OMTs) different plant species; the highest identity (41%) observed putative OMT expressed in roots maize. phylogenetic analysis that and maize sequences are distinctly ortho-diphenol-OMTs involved lignin formation. S-adenosyl-L-methionine-binding motif (KELVDDSITN) determined for rabbit protein-carboxyl is partially conserved encoded acid sequence. Genomic Southern blot hybridization probably represents single copy gene. F1 clone corresponds gene transcript exhibiting relatively late accumulation mildew-infected compared other pathogen-induced transcripts, such as transcripts PR proteins, peroxidase, homologous caffeic OMT. No detected plants papilla resistance at time points when thought be manifested. atypical pattern also infection by pathogens UV-light treatment.