Activation of the trpBA promoter of Pseudomonas aeruginosa by TrpI protein in vitro.

摘要: We have developed an in vitro transcription system which purified TrpI protein and indoleglycerol phosphate (InGP) activate initiation at the trpBA promoter (trpPB) repress trpI (trpPI) of Pseudomonas aeruginosa. The phenotypes resulting from mutations -10 region both promoters indicate that consensus sequence P. aeruginosa is probably same as Escherichia coli. Furthermore, absence InGP, activities two are inversely correlated: down trpPI lead to increased activity trpPB, up trpPB cause a decrease activity. These results consequence fact overlap, so RNA polymerase cannot form open complexes with simultaneously. Thus, theory, by preventing binding trpPI, could indirectly trpPB. However, oligonucleotide-induced completely inactivate do not relieve requirement for InGP Therefore, activation mediated direct effect on In addition, alter site II, weaker sites identified DNase I hydroxyl radical footprinting studies (M. Chang I. Crawford, Nucleic Acids Res. 18:979-988, 1990). Since these prevent full we conclude specific base pairs II required activation. Images