[19] d-glucose-6-phosphate

摘要: Publisher Summary Fructose-6-phosphate can converted to glucose-6-phosphate by the action of hexose phosphate isomerase. In presence barium ion, may be obtained acid hydrolysis fructose-l,6-diphosphate. Active preparations isomerase prepared from rat or rabbit skeletal muscle bacterial extracts. The enzyme must freed sulfate and ions dialysis. A sensitive highly specific method for determination is reduction triphosphopyridine nucleotide (TPN) in dehydrogenase. amount calculated change optical density at 340 mμ. purity crystalline salt 98 102% this enzymatic assay procedure without recrystallization. Unlike amorphous product, heptahydrate insoluble water. solubility approximately 2×l0 -8 M. 25 ° 1×10 -3 0°. It readily soluble dilute acid.