Combination of endolysins and high pressure to inactivate Listeria monocytogenes.

摘要: Outbreaks of listeriosis are often related to the consumption low-processed ready-to-eat food products (e.g. soft cheeses or smoked fish) contaminated with Listeria monocytogenes. Traditional preservation techniques, such as heat treatment, cannot eliminate from these without strongly affecting quality foods. We therefore investigated use endolysin (PlyP40, Ply511, PlyP825) in combination high hydrostatic pressure processing kill L. monocytogenes buffer. The results demonstrated a more than additive effect when both treatments were combined. For example, whereas 0.16 μg/mL PlyP825 300 MPa (1 min, 30 °C) applied individually reduced cell count by 0.2 and 0.3 log cfu, respectively, combined treatment resulted reduction 5.5 cfu. Similar obtained for other endolysins processing. also showed that synergistic inactivation cells HHP is possible at level only 200 (2 °C). Thus, application did not substantially increase bactericidal pressure, but it enabled bacterial much lower levels. This shows potential using processes preservation.