Minimal Residual Disease Detection by Droplet Digital PCR in Multiple Myeloma, Mantle Cell Lymphoma, and Follicular Lymphoma: A Comparison with Real-Time PCR
作者： Daniela Drandi , Lenka Kubiczkova-Besse , Simone Ferrero , Nadia Dani , Roberto Passera
DOI: 10.1016/J.JMOLDX.2015.05.007
关键词: Lymphoma 、 Digital polymerase chain reaction 、 Multiple myeloma 、 Follicular lymphoma 、 Mantle cell lymphoma 、 Molecular biology 、 Immunoglobulin gene 、 Biology 、 Real-time polymerase chain reaction 、 Minimal residual disease
摘要: Real-time quantitative PCR (qPCR) is a well-established tool for minimal residual disease (MRD) detection in mature lymphoid malignancies. Despite remarkable sensitivity and specificity, qPCR has some limitations, particularly the need reference standard curve, based on target serial dilutions. In this study, we established droplet digital (ddPCR) MRD monitoring multiple myeloma, mantle cell lymphoma, follicular lymphoma compared it head-to-head with qPCR. We observed that ddPCR sensitivity, accuracy, reproducibility comparable then two approaches 69 patients documented molecular marker at diagnosis (18 myelomas, 21 lymphomas assessed immunoglobulin gene rearrangement, 30 use of BCL2/immunoglobulin major breakpoint region rearrangement). was successful 100% cases, whereas failed to provide reliable curve three patients. Overall, 222 225 samples were evaluable by both methods. The comparison highlighted good concordance (r = 0.94, P
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