Detection and Quantification of BCR-ABL1 Fusion Transcripts by Droplet Digital PCR

作者: Lawrence J. Jennings , David George , Juliann Czech , Min Yu , Loren Joseph

DOI: 10.1016/J.JMOLDX.2013.10.007

关键词:

摘要: … Herein, we demonstrate the advantages of RT-ddPCR for quantifying BCR-ABL1 fusion transcripts as compared to the widely adopted standard method, RT-qPCR. The most important …

参考文章(45)
M J Brisco, A A Morley, P J Sykes, E Hughes, J Condon, S H Neoh, Quantitation of targets for PCR by use of limiting dilution. BioTechniques. ,vol. 13, pp. 444- 449 ,(1992)
Nóra Faragó, Ágnes K. Kocsis, Sándor Lovas, Gábor Molnár, Eszter Boldog, Márton Rózsa, Viktor Szemenyei, Enikő Vámos, Lajos I. Nagy, Gábor Tamás, László G. Puskás, Digital PCR to determine the number of transcripts from single neurons after patch-clamp recording BioTechniques. ,vol. 54, pp. 327- 336 ,(2013) , 10.2144/000114029
Richard D. Press, Zac Love, Ashlie A. Tronnes, Rui Yang, Thuan Tran, Solange Mongoue-Tchokote, Motomi Mori, Michael J. Mauro, Michael W. Deininger, Brian J. Druker, BCR-ABL mRNA levels at and after the time of a complete cytogenetic response (CCR) predict the duration of CCR in imatinib mesylate-treated patients with CML. Blood. ,vol. 107, pp. 4250- 4256 ,(2006) , 10.1182/BLOOD-2005-11-4406
Helen E. White, Paul Matejtschuk, Peter Rigsby, Jean Gabert, Feng Lin, Y. Lynn Wang, Susan Branford, Martin C. Müller, Nathalie Beaufils, Emmanuel Beillard, Dolors Colomer, Dana Dvorakova, Hans Ehrencrona, Hyun-Gyung Goh, Hakim El Housni, Dan Jones, Veli Kairisto, Suzanne Kamel-Reid, Dong-Wook Kim, Stephen Langabeer, Edmond S. K. Ma, Richard D. Press, Giuliana Romeo, Lihui Wang, Katerina Zoi, Timothy Hughes, Giuseppe Saglio, Andreas Hochhaus, John M. Goldman, Paul Metcalfe, Nicholas C. P. Cross, Establishment of the first World Health Organization International Genetic Reference Panel for quantitation of BCR-ABL mRNA. Blood. ,vol. 116, pp. 111- 117 ,(2010) , 10.1182/BLOOD-2010-06-291641
N. Takahashi, T. Kyo, Y. Maeda, T. Sugihara, K. Usuki, T. Kawaguchi, N. Usui, S. Okamoto, Y. Ohe, S. Ohtake, K. Kitamura, M. Yamamoto, H. Teshima, T. Motoji, T. Tamaki, K. Sawada, K. Ohyashiki, Discontinuation of imatinib in Japanese patients with chronic myeloid leukemia Haematologica. ,vol. 97, pp. 903- 906 ,(2012) , 10.3324/HAEMATOL.2011.056853
Susan Branford, Linda Fletcher, Nicholas C. P. Cross, Martin C. Müller, Andreas Hochhaus, Dong-Wook Kim, Jerald P. Radich, Giuseppe Saglio, Fabrizio Pane, Suzanne Kamel-Reid, Y. Lynn Wang, Richard D. Press, Kevin Lynch, Zbigniew Rudzki, John M. Goldman, Timothy Hughes, Desirable performance characteristics for BCR-ABL measurement on an international reporting scale to allow consistent interpretation of individual patient response and comparison of response rates between clinical trials Blood. ,vol. 112, pp. 3330- 3338 ,(2008) , 10.1182/BLOOD-2008-04-150680
David George, Juliann Czech, Bobby John, Min Yu, Lawrence J. Jennings, Detection and quantification of chimerism by droplet digital PCR Chimerism. ,vol. 4, pp. 102- 108 ,(2013) , 10.4161/CHIM.25400
Izhar Hardan, Anfisa Stanevsky, Yuliya Volchek, Tali Tohami, Ninette Amariglio, Luba Trakhtenbrot, Maya Koren-Michowitz, Avichai Shimoni, Arnon Nagler, Treatment with interferon alpha prior to discontinuation of imatinib in patients with chronic myeloid leukemia. Cytokine. ,vol. 57, pp. 290- 293 ,(2012) , 10.1016/J.CYTO.2011.11.018
Elia Mattarucchi, Orietta Spinelli, Alessandro Rambaldi, Francesco Pasquali, Francesco Lo Curto, Leonardo Campiotti, Giovanni Porta, Molecular Monitoring of Residual Disease in Chronic Myeloid Leukemia by Genomic DNA Compared with Conventional mRNA Analysis The Journal of Molecular Diagnostics. ,vol. 11, pp. 482- 487 ,(2009) , 10.2353/JMOLDX.2009.080150
Richard D. Press, Stephanie G. Willis, Jennifer Laudadio, Michael J. Mauro, Michael W. N. Deininger, Determining the rise in BCR-ABL RNA that optimally predicts a kinase domain mutation in patients with chronic myeloid leukemia on imatinib. Blood. ,vol. 114, pp. 2598- 2605 ,(2009) , 10.1182/BLOOD-2008-08-173674