Digital PCR to determine the number of transcripts from single neurons after patch-clamp recording

作者: Nóra Faragó , Ágnes K. Kocsis , Sándor Lovas , Gábor Molnár , Eszter Boldog

DOI: 10.2144/000114029

关键词:

摘要: … To circumvent the sensitivity issues associated with single cell qRT-PCR, we used high-throughput nanocapillary qRT-PCR to record digital signals from mRNA isolated from a single …

参考文章(28)
Yong Zeng, Richard Novak, Joe Shuga, Martyn T. Smith, Richard A. Mathies, High-Performance Single Cell Genetic Analysis Using Microfluidic Emulsion Generator Arrays Analytical Chemistry. ,vol. 82, pp. 3183- 3190 ,(2010) , 10.1021/AC902683T
J. Eberwine, H. Yeh, K. Miyashiro, Y. Cao, S. Nair, R. Finnell, M. Zettel, P. Coleman, Analysis of gene expression in single live neurons. Proceedings of the National Academy of Sciences of the United States of America. ,vol. 89, pp. 3010- 3014 ,(1992) , 10.1073/PNAS.89.7.3010
Fuchou Tang, Petra Hajkova, Sheila C Barton, Dónal O'Carroll, Caroline Lee, Kaiqin Lao, M Azim Surani, 220-plex microRNA expression profile of a single cell Nature Protocols. ,vol. 1, pp. 1154- 1159 ,(2006) , 10.1038/NPROT.2006.161
Christopher J Price, Bruno Cauli, Endre R Kovacs, Akos Kulik, Bertrand Lambolez, Ryuichi Shigemoto, Marco Capogna, Neurogliaform Neurons Form a Novel Inhibitory Network in the Hippocampal CA1 Area The Journal of Neuroscience. ,vol. 25, pp. 6775- 6786 ,(2005) , 10.1523/JNEUROSCI.1135-05.2005
Sandra L. Spurgeon, Robert C. Jones, Ramesh Ramakrishnan, High Throughput Gene Expression Measurement with Real Time PCR in a Microfluidic Dynamic Array PLoS ONE. ,vol. 3, pp. e1662- ,(2008) , 10.1371/JOURNAL.PONE.0001662
Brooke H. Miller, Claes Wahlestedt, MicroRNA dysregulation in psychiatric disease. Brain Research. ,vol. 1338, pp. 89- 99 ,(2010) , 10.1016/J.BRAINRES.2010.03.035
Martin Bengtsson, Anders Ståhlberg, Patrik Rorsman, Mikael Kubista, Gene expression profiling in single cells from the pancreatic islets of Langerhans reveals lognormal distribution of mRNA levels Genome Research. ,vol. 15, pp. 1388- 1392 ,(2005) , 10.1101/GR.3820805