Mitochondrial DNA control region as a tool for species identification and distinction between wolves and dogs from Croatia.

作者: Magda Sindičić , Josip Kusak , Đuro Huber , Tomislav Gomerčić , Ana Galov

DOI:

关键词: HaplotypeHybridGeneticsmtDNA control regionWildlife conservationEvolutionary biologyBase pairGeneNuclear geneBiologyMitochondrial DNA

摘要: Dogs worldwide share a single genetic origin from Asian wolves. While dog and wolf lineages are diffi cult to separate in terms of nuclear genes, mitochondrial clearly distinguishable for the two species, offering good opportunity evaluate differences between them. Species identifi cation DNA is an important tool conservation Croatia, differentiation necessary forensic cases, wildlife management scientifi c research. The goal this paper was provide data set on Croatian control region sequences, research if these sequences can be used as reference species cation. We analyzed 281 base pair (mtDNA) 20 mixed breed blood samples, 91 grey muscle samples wolf-like animals. ed 12 4 mtDNA haplotypes. None haplotypes were shared, confi rming that discriminate wolves dogs, rm maternal ancestry putative hybrids. animals grouped into cluster.

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